Fig. 1
From: Chitosan-GSNO nanoparticles: a positive modulator of drought stress tolerance in soybean
Characterization of chitosan nanoparticles and chitosan-GSNO nanoparticles. The CSNPs and CS-GSNONPs were characterized via scanning electron microscopy showing agglomerated morphology of CSNP (A) and spherical morphology of CS-GSNONPs (B). The nanoparticles were characterized using FTIR analysis detecting three unique peaks with wavenumbers of 3343 cm−1, 2920 cm−1, and 1590 cm−1 for pure chitosan and CSNPs (C). These peaks were also detected for CS-GSNONPs but with a significantly lower absorbance (D). Further characterization via XRD analysis also indicated unique characteristics for pure chitosan and chitosan-GSNO nanoparticles (E). Analysis of CS-GSNONPs for temporal kinetics of spontaneous gaseous NO release via Nitric Oxide analyzer (NOA280i, SIEVERS, USA) indicated a significantly slow and long-term release of NO as compared to free GSNO (F)