Fig. 5

Correlations between the average number of nucleotide differences (K) and GC content (G-C frequency) among 165 ITS2 sequence-structure matrices. Each data point represents the average GC value or G-C frequency for a single genus. The regression line was calculated using Pearson’s correlation, with the error bands represent 95% confidence intervals based on a binomial model. A Comparison of the GC content and the K value for the entire ITS2 sequence. B Comparison of the GC content and the K value for the ITS2 paired regions. C Comparison of the G-C frequency and the K value for the ITS2 paired regions. D Comparison of the GC content and the K value for the ITS2 unpaired regions