Fig. 4

Effect of Ca limitation on L. chinensis root membrane selectivity. Plant materials were grown as in Fig. 1; the imaging procedure was as in Fig. 3. (A) Alexa Fluor 488, a membrane-impermeable green fluorescent dye without biological activity, was used to track specific fluid-phase-based endocytosis. L. chinensis seedlings were co-stained with FM4-64 and Alexa Fluor 488 for 30 min. (B) Tyr A23, an inhibitor of receptor-mediated endocytosis, and Tyr A51, a structural analog of Tyr A23 without inhibitory activity, served as the control. L. chinensis seedlings were treated with 30 µM Tyr A23 or Tyr A51 in corresponding solution for 1 h and then dyed for 30 min. Scale bars, 50 μm