Fig. 3

Pollen performance among self- and cross-pollinations in S-gene homozygote germplasm. A Micrographs showing pollen performance under self-pollination and cross-pollination in ‘S₁S₂’, ‘S₁S₁’ and ‘S₂S₂’. Arrow indicates the longest pollen tube in the pistil. Bar = 100 mm. B Pollen tube lengths relative to pistil length. Data are mean values and standard errors of three replicates. Different letters indicate significant differences as determined by ANOVA followed by Tukey’s multiple comparison test (P < 0.05). C Typical images of S-gene homozygote germplasm pollen tubes under in vitro culture conditions with different S-proteins (1.25 μg·μL^–1). Bar = 100 μm. D Analyses of S-gene homozygote germplasm pollen tube length. At least 500 measurements were performed. Effect of S-proteins on pollen germination and growth of pollen tubes after 12 h of culture. Different letters indicate significant differences as determined by ANOVA followed by Tukey’s multiple comparison test (P < 0.05)