Fig. 8

The subcellular localization and transcriptional activity analysis of five GATA genes. A Illustration of the constructs used for the subcellular localization and transcriptional activity assays. B Subcellular localization of five GATA proteins. The 35S-VamGATAs-GFP and 35S-GFP control were transiently expressed in Nicotiana benthamiana leaf epidermal cells. The Arabidopsis thaliana 35S-AtHY5-mCherry was chosen as a nuclear localization marker gene [37]. Scale bar = 40 μm. C Transactivation activity assay of five GATA proteins in yeast. The transformed yeast cells were grown on SD/-Trp/X-α-gal/AbA medium and blue color indicate transcriptional activity. pGBKT7-53 co-transformed with pGADT7-T was used as the positive control, and pGBKT7-Lam co-transformed with pGADT7-T was used as the negative control