Fig. 7
qRT-PCR confirmation of in silico gene expression. a qRT-PCR results showing expression of genes taken from RNA sampled from 3 pooled biological replicates of different sweet potato plant parts. The bars represent the mean ± standard error (SE) (n = 3). GS: green stem; PR: pencil root; SR: storage root; FR: fibrous root; L: leaf. Bars not sharing a common letter showed significant differences in gene expression using the Kruskal–Wallis H test (p < 0.05) b Comparison of log2FC data for SR vs. FR from in silico and qRT-PCR gene expression data. Asterisks indicate statistically significant (p-value < 0.05) log2FC (found using DESeq2 for in silico data and Student’s t-test for qRT-PCR data). A single asterisk indicates statistically significant log2FC from the RNA-seq data only, while 2 asterisks indicate statistically significant log2FC for both the RNA-seq and qRT-PCR data. The error bars for the qRT-PCR data represent the confidence intervals derived from the mean ± SE of the ΔΔCT values (n = 3)