Fig. 4

Phylogenetic tree, conserved motif, and gene structure of MsWRKY family members. a Phylogenetic tree of MsWRKY genes was constructed using MEGA-X and the neighbor-joining (NJ) method. b Conserved motif of MsWRKY proteins was analyzed on the MEME tool, and the results were visualized in TBtools. The motifs, labeled as 1–8 and represented by different colored boxes, demonstrate the conserved patterns in the protein sequences. The scale at the bottom allows for estimation of protein lengths. c The gene structure of MsWRKY genes was determined, with the coding regions for the MsWRKY domain highlighted in yellow. Regions labeled as CDS without the region coding for the MsWRKY domain, indicated in green