Fig. 6

Endosperm-Embryo hypomethylated DMR analysis in WT and h2a.x mutants. Analysis of h2a.x methylomes, comparing DMRs between endosperm and embryo. (a) Venn diagram illustrating that WT embryo and endosperm harbour 4451 DMRs, the majority of which (3238) are shared with h2a.x embryo and endosperm. h2a.x embryo and endosperm have an additional 4357 DMRs. (b) Box plots showing the relative methylation level of DMRs in embryo and endosperm, in wild type, h2a.x and dme-2 mutants. (c) Characterization of h2a.x-specific embryo-endosperm DMRs; wild-type and h2a.x Endosperm-Embryo DMRs grouped by size, with the cumulative total length they covered shown, whereby they are represented across all DMR sizes, and represent an overall increase in size distribution. (d) Fractional methylation difference between h2a.x mutant and WT CG methylation from the maternal endosperm allele is plotted, data in 50 bp windows with > 20 × sequence coverage, for all loci (green), DME target loci (red) and DME-FACT target loci (orange)