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Fig. 4 | BMC Plant Biology

Fig. 4

From: A rare non-canonical splice site in Trema orientalis SYMRK does not affect its dual symbiotic functioning in endomycorrhiza and rhizobium nodulation

Fig. 4

Parasponia symrk-5 mutant trans-complementation of root nodule symbiosis. A Schematic representation of P. andersonii SYMRK gene structure. Arrowhead points to the location of the introduced GA mutation in PanSYMRK at the 5’-donor splice site of intron 12. B Nodule number per plant formed on Pansymrk-5 A. rhizogenes transformed root with pPanSYMRK:PanSYMRK gene (n = 13). (C-E) Nodule number per plant (n = 5) C, representative image of green flurescence protein (GFP) nodule D and a section through a mature nodule E of Pansymrk-5 A. rhizogenes transformed root with pPanSYMRK:PanSYMRKGA carrying a GA mutation at the 5’-donor splice site of intron 12. Nodules were harvested and analysed at 8 weeks post inoculation with Mesorhizobium plurifarium BOR2 (OD600 = 0.025)

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BMC Plant Biology

ISSN: 1471-2229

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