Fig. 7

Effect of LbMAPK2 silenceing on salt gland development and salt secretion of L. bicolor.(A) Distribution and salt secretion of salt glands in different lines leaves for control group and experimental group, the scale bar of leaf disc = 2.5 mm, the scale bar of salt gland density = 50 μm. (B) The area of a single leaf of TRV::0 for control group and TRV::LbMAPK2 of different lines for experimental group. (C) The number of salt glands in TRV::0 leaf disc for control group, TRV::LbMAPK2 leaf disc for experimental group. (D) 24-h secretion of leaf disc of TRV::0 for control group, TRV::LbMAPK2 of different lines for experimental group. (E) Representative images of the non-invasive electrode used for NMT. (F) The Na⁺ efflux in including empty vector (TRV::0) and silenced lines of LbMAPK2. Net Na⁺ efflux rate per single salt gland of different lines over 180 s. (G) Average net Na⁺ efflux rate per salt gland in different lines. The data are the mean of the Na⁺ efflux based on the 180 s time course from three salt glands. All of the above data were set with three replicates and standard deviation (SD) was calculated to ensure accuracy. Letters above the bar (a-f) were used to indicate significant differences between different columns (p = 0.05, Duncan)