Fig. 9
From: Functional and kinetics of two efficient phenylalanine ammonia lyase from Pyrus bretschneideri
Yeast one-hybrid assay to verify the binding activity of PbWLIM1 and PAL-box element. Note: A PbWLIM1 can bind to the conserved PAL box in the PbPAL promoter, 1: Stock solution, 10–1, 10–2 and 10–3: the bacterial solution is diluted 10, 100 and 1000 times; B Detection of the activation activity of pGBKT7-PbWLIM1a/b in yeast, 1,2,3,4 represents the adjustment of 4 bacterial plaques, + and—represent positive and negative controls respectively, 1, 10–2 and 10–3: the bacterial solution is diluted 10, 100 and 1000 times