Fig. 7
From: Identification of HpMYB1 inducing anthocyanin accumulation in Hippeastrum Hybridum tepals by RNA-seq
Sequence, expression patterns and subcellular localization analysis of HpMYB1. (A) Phylogenetic tree of HpMYB1 and 22 other known anthocyanin-related R2R3-MYBs in other plants. (B) Multiple alignment of the conserved R2-domain and R3-domain of different anthocyanin biosynthesis related R2R3-MYB proteins. The black line showed the conserved R2 and R3 domain, The red line indicated a motif ([D/E]Lx2[R/K]x3Lx6Lx3R) interacting with a bHLH TF. (C) The anthocyanin content and the relative expression of HpMYB1 at different tepal developmental stages and different tissues. (D) Subcellular localization of HpMYB1 protein in Arabidopsis thaliana mesophyll protoplasts. Bar = 5 μm, YFP: yellow fluorescence protein; mCherry: nuclear localization