Fig. 5
From: Identification of HpMYB1 inducing anthocyanin accumulation in Hippeastrum Hybridum tepals by RNA-seq
qRT-PCR verifcation of the expression profles of selected unigenes involved in anthocyanin biosynthesis. Twelve unigenes from the anthocyanin biosynthesis pathway were used to validate the RNA-seq results. The expression levels were calculated by the 2−∆∆CT method. The y-axis on the left represents the unigene relative expression by qRT-PCR and the y-axis on the right indicates the TPM value of the unigene in RNA-seq data. The unigene ID and specific primers were provided in Supplementary Table S5