Table 2 Differentially expressed genes in the WT and Csbpc2 mutants used for analysis of qRT‒PCR
From: CsBPC2 is essential for cucumber survival under cold stress
Gene ID | WT-log2 fold Change | L2-log2 fold Change | L3-log2 fold Change | WT-regulation | L2-regulation | L3-regulation | Forward primer Sequence (5'-3') | Reverse primer Sequence (5'-3') |
|---|---|---|---|---|---|---|---|---|
Csa3G598900 | 1.160573749 | 0.71314422 | 0.970121949 | up | up | up | TTTAGGCGGTGAAAATGGTC | CACAGGCATCGAAGTTCTCA |
Csa1G459500 | 0.485592215 | 0.148236934 | 0.037536613 | up | up | up | GTTGTGCTCCGTCTCTTCTT | TCCGATGGGATCGTTTCTTATG |
Csa3G732550 | -0.760215992 | -0.103116865 | -0.022952602 | down | down | down | AGAAGTCATTTGTGACAATGGC | CAATACCAATAGCTCCGACTCT |
Csa5G202330 | -1.089579003 | -1.509829003 | -1.514338257 | down | down | down | TCTTCATCATCTTGTCACCCAA | TCGTTCTTACGCGTCACATATA |
Csa6G501930 | 1.271882853 | 1.057699327 | 1.258896149 | up | up | up | CGAAGCAAAGAAGCAAAACATC | TCAAGCTAAAATGTTCGGTTCG |
Csa2G361450 | 0.364259096 | 0.216829221 | 0.243954024 | up | up | up | CTCTCTCTCTTTCCCAATCGAA | CGATTCTTCAGTTCCGACAATC |
Csa3G643770 | 0.777739139 | 1.741103697 | 1.712188562 | up | up | up | GGAGAGTTCAGCCGTGACAGTAGA | ACACATCCTTCTTCCTGACCCAAATG |
Csa7G407550 | 0.803906259 | 1.796374033 | 1.798755046 | up | up | up | CGATAACTCCGTCTCCGTCCTCTT | GAACAACTCTCCGATCAACCTCACTC |
Csa1G533380 | -0.631431667 | -1.412083745 | -1.363809409 | down | down | down | TTGGTGTGATTCAAGGCTCTAT | TCCGTCGCCATATAAGCATAAT |
Csa2G369740 | -0.765350909 | -1.584917728 | -1.810774018 | down | down | down | AACAAAAGTTCACGAGCTACAC | CTGCTATGTACTCTCCATTCGT |
CsActin | / | / | / | / | / | / | TTCTGGTGATGGTGTGAGTC | GGCAGTGGTGGTGAACATG |