Wheat stripe rust resistance locus YR63 is a hot spot for evolution of defence genes – a pangenome discovery

BMC Plant Biology

Table 2 KASP markers used to map YR63 on chromosome arm 7BS

Marker	Position^a	Allele 1 Specific Forward Primer	Allele 2 Specific Forward Primer	Common Reverse Primer
sunKASP_401	262,576	ATGTTGTGTAGAAATTAGAGAATATGGAGT	GTTGTGTAGAAATTAGAGAATATGGAGC	CACGTGTTCAGCAAAAGGAG
sunCS_YR63	904,156	CTGAATCACATCTATTAACCTCCAAATC	CTGAATCACATCTATTAACCTCCAAATG	AAGTTTGTGACTGCCCCAAGAT
sunCS_67	4,028,196	GCACCGTTGGTACTATTTAGCAT	GCACCGTTGGTACTATTTAGCAC	CCCCAAGCTTGCTACAGTGTC
sunCS_36	5,843,626	AGCTGTAAATAATTGCCTCACCT	AGCTGTAAATAATTGCCTCACCC	GCTACGCGGAAATTTGACCA
sunKASP_406	7,984,146	TGCCATCTAGTTGAGTAACCTCTG	AATGCCATCTAGTTGAGTAACCTCTA	CACAAAAACCCCTTCACACC
sunKASP_409	13,227,705	CAATGCATTTTCTTCTTCTCCG	CAATGCATTTTCTTCTTCTCCC	CTTCACCACCGCATTCCTA
sunKASP_407	13,398,475	AATTGCCCAAGAGGGTCTAA	AATTGCCCAAGAGGGTCTAG	GCGTTTGGGTATCATTCCAC

^aBased on Chinese Spring (v2.1). Allele 1 primer synthesised with FAM: GAA GGT GAC CAA GTT CAT GCT; Allele 2 primer synthesised with HEX: GAA GGT CGG AGT CAA CGG ATT

ISSN: 1471-2229