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Table 2 Probit analysis to determine the lethal dose (LD 50) of various EMS concentrations exposed at 2 h, 4 h 6 h, and 8 h based on mortality rate in Choy Sum

From: EMS-induced mutagenesis in Choy sum (Brassica chinensis var. parachinensis) and selection for low light tolerance using abiotic stress indices

Corrected mortality

Concentration (%)

Log Dose

Observed mortality

Empirical Probits

LC50 (%)

[Min -Max]

Slope

Intercept

Chi-Square (df = 4)

2 h

0

0.00

0

0

17.29 (5.74—52.03)

0.995

3.768

0.04

0.1

-1.00

2

2.95

0.3

-0.52

3

3.12

0.5

-0.30

4

3.25

1

0.00

8

0.00

1.5

0.18

10

3.72

2

0.30

29

4.45

4 h

0

0.00

0

0.00

1.55 (0.96—2.51)

1.759

4.663

0.678*

0.1

-1.00

2

2.95

0.3

-0.52

13

3.87

0.5

-0.30

14

3.92

1

0.00

33

0.00

1.5

0.18

48

4.95

2

0.30

64

5.36

6 h

0

0.00

0

0.00

0.97 (0.62—1.50)

1.86

5.070

0.164*

0.1

-1.00

3

3.12

0.3

-0.52

26

4.36

0.5

-0.30

35

4.61

1

0.00

38

0.00

1.5

0.18

52

5.05

2

0.30

86

6.08

8 h

0

0.00

0

0.00

0.54 (0.36—0.84)

1.93

5.533

0.766*

0.1

-1.00

7

3.52

0.3

-0.52

36

4.64

0.5

-0.30

52

5.05

1

0.00

64

0.00

1.5

0.18

82

5.92

2

0.00

100

0.00

  1. *Significant difference at P < 0.05