Table 1 Phytochrome primers used for PCR amplification
S.no | Primer | Direction | Sequence (5' → '3') | Size (bp) |
|---|---|---|---|---|
1 | PhyA | Forward (F) | ATCATTGCACAGACCACCGT | 1190 |
Reverse (R) | CATCGCGCATCAGCATATCG | |||
2 | PhyB | Forward (F) | CGACTGGTTTAAGCACGGATAG | 456 |
Reverse (R) | CCAGCATCCACAGCGAATATAG | |||
3 | PhyC | Forward (F) | CCGCCGTTTGTGGATAATAGAG | 834 |
Reverse (R) | GAACCTGTAGAGCGTATTGGAG | |||
4 | PAR1 | Forward (F) | TCTCTCTCTCTCTCACACACAC | 250 |
Reverse (R) | GCCGTTCCTCCGGGAATAAT | |||
5 | PAR2 | Forward (F) | GACGCGCTGTTTGAAGAGAC | 255 |
Reverse (R) | GCAAAAGAGCCAGCCACAAT |