Fig. 5

Effects of GA or C646 on lipid breakdown in maize aleurone layers in the absence or presence of RG108. (A) qRT-PCR was used to verify the detailed expression profiles of genes involved in lipid catabolism. (B) Detection of endogenous lipid contents in aleurones after treatment with GA, C646, GA plus RG108 or C646 plus RG108 as compared with the control. The x-axis indicates the different time points; the y-axis indicates relative expression values or neutral lipid contents. Each assay was repeated three times for every sample in three independent experiments. ns indicates not significant, asterisks indicate statistically significant differences. *, P < 0.05, ***, P < 0.01, as compared with the control with the Student’s t test