Fig. 4

Analysis of effects of the DNA methylation inhibitor RG108 on GA or C646 mediated PCD in aleurones monitored by FDA/PI staining. (A) The addition of RG108 suppressed GA or C646 induced PCD in maize aleurones. Bar = 100 μm. (B) Dot blot analysis of DNA methylation levels after treatment with GA or C646 in the absence or presence of DNA methylation inhibitor RG108. The mean gray value for DNA methylation after treatment with RG108 was significantly reduced compared with the value of the control. Each assay was repeated three times for every sample in three independent experiments. *, P < 0.05, ***, P < 0.01, by the Student’s t test