Fig. 5

AtNOL12 affects pre-rRNA processing under heat stress. Northern blot analysis of mRNA (A) and rRNA precursors and intermediates (B) in WT, nol12-4 and 35S::NOL12-GFP/nol12 lines. Samples were collected from 14-day-old seedlings and transferred to 37°C for the times indicated. RNA was separated on 1.1% agarose gels and hybridized with HSP70 (A) and probes P5, P23, P42, and P6 (B). rRNA precursors and intermediates are described on the right. Black arrowheads indicate the heat stress-dependent species, including the P-C2 intermediate, migrating just above the P-A3 [46], asterisk indicates a nol12-specific intermediate that probably corresponds to 5.S-3′ species. The values provided above or below RNA intermediates show changes in their accumulation in the mutants compared to WT; numbers in bold represent relative values in the mutants compared to the WT at the corresponding treatment time, the remaining numbers denote relative values compared to the untreated plants (NT) for the same line. U2 snRNA was used as a loading control (LC). The analyses were performed in three biological replicates. Full-length blots are included in a Supplementary Information file