Fig. 4
The nol12-4 mutant is sensitive to Pseudomonas syrignae pv. tomato DC3000 infection. (A) Growth of Pst DC3000 after 24 and 72 hpi in WT and the nol12-4 mutant. Results are the mean of four independent experiments and error bars represent SD; P < 0.01 (Student’s t-test). Lower panel: images of indicated plants infected with Pst DC3000 and control plants treated with MgCl2 (B) Northern blot analysis of factors involved in response to Pst DC3000. Samples were collected from non-treated (NT), control (MgCl2) and infected (Pst) WT and nol12-4 plants at indicated time points. The ratio of transcript level in treated nol12-4 versus WT normalized to 18S rRNA loading control (LC) is shown as the main numbers, while the ratio relative to the control conditions is given in italics. (C) Northern blot analysis of factors involved in PAMPs response. Samples were collected at indicated time points from non-treated (NT) 14-day-old seedlings, treated with MS (control) or 100 nM of flg22 and elf18. The ratio of transcript level in treated nol12-4 versus WT normalized to 18S rRNA loading control (LC) is shown as the main numbers, while the ratio relative to the control conditions is given in italics. (D-E) Northern blot analysis of pre-rRNA precursors in WT and nol12-4 lines using probes P5 and P42. Numbers represent the ratio of transcript level in Pst-treated WT and the mutant relative to control (MgCl2) and normalized to 18S rRNA loading control (LC). Northern analyses in (B-E) were performed in at least three biological replicates. Full-length blots are included in a Supplementary Information file