Fig. 3

The nol12-4 mutant accumulates rRNA precursors of the large ribosomal subunit. (A) Schematic representation of pre-rRNA structure and processing intermediates. Localization of probes used for northern blot analysis is shown. (B-C) Northern blot analysis of high-molecular weight (B) and low-molecular-weight (C) pre-rRNA precursors in WT and nol12-4 mutant plants using probes depicted in (A). RNA was separated on 1.1% agarose or 6% acrylamide gels. rRNA precursors and intermediates are indicated on the right. Two large pre-rRNAs, 35S-P and 33S-P′, are not distinguished by northern analysis. Probes for mature 18S rRNA (B) and 7SL RNA (C) were used as loading controls (LC). Schematic representation of detected low-molecular-weight pre-rRNAs is shown in (C). Northern blots were performed in at least three biological replicates. (D) Primer extension analysis for 5.8S and 25S 5′ ends. Full-length gels and blots are included in a Supplementary Information file