Fig. 1

AtNOL12 is a conserved nucleolar protein. (A) Fragment of T-Coffee sequence alignment of NOL12 homologues. AtNOL12 (Arabidopsis thaliana), HsNOL12 (human), RnNop25 (Rattus norvegicus), DmViriato (Drosophila melanogaster) and ScRrp17 (yeast Saccharomyces cerevisiae). The putative catalytic domain is indicated according to [16, 19]. (B) Structure of the AtNOL12 (At1G11240) gene. Exons are represented with grey bars and localization of T-DNA insertions are indicated. (C) Northern blot analysis of NOL12 mRNA in wild-type (WT) nol12-3 and nol12-4 mutant lines. Hybridization for EIF4A1 mRNA was used as a loading control (LC). Full-length blots are included in a Supplementary Information file. Analysis was performed in two biological replicates. (D-H) The morphological phenotype of nol12-4 plants. Scale bars represent 1 cm (D-F) or 1 mm (G-H). (I) Nucleolar localization of NOL12 fused to GFP observed by confocal microscopy in Arabidopsis root hair cells. Scale bar represents 10 μm