Fig. 5From: Identification of novel flavin-dependent monooxygenase from Strobilanthes Cusia reveals molecular basis of indoles’ biosynthetic logicExpression of recombinant ScFMO and indigo production. (A) After induced by IPTG, the color of the culture was observed to change over time. The 2 and 4 shows E. coli harboring pET28a or pET28a-ScFMO without substrates that had been cultured for 24 h. (B) Linear regression curves of indigo by microplate absorbance reader with 630 nm filters. (C) Indigo production from indole and tryptophan in the culture over timeBack to article page