Fig. 2

Identification and characterization of induced MLO mutations in T0 transgenic soybean plants. A Gel electrophoresis of PCR-amplicons of GmMLO target expanding regions. WT, non-transgenic wild-type plant; 3.1, 15.1, transgenic lines; M, 1 kb DNA ladder. B-G Sequence alignment of targeted regions in GmMLO genes (GmMLO02, GmMLO10, GmMLO18, GmMLO19, GmMLO20 and GmMLO23) of T0 transgenic lines. Target sequences and PAMs are indicated in red and blue color, respectively. Inserted nucleotides are shown in yellow. a/b/c/d/e indicates different alleles for each T0 line; ∆ indicates targeted sequence changes: 0 for no change, -for deletion, + for insertion. Clones indicate number of colonies with the respective alleles out of total of clones sequenced