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Fig. 1 | BMC Plant Biology

Fig. 1

From: Enhancing powdery mildew resistance in soybean by targeted mutation of MLO genes using the CRISPR/Cas9 system

Fig. 1

GmMLO gene maps, gRNA sequences, target locations and CRISPR/Cas9 vector. A gRNA and PAM sequences. B GmMLO genes and target sequences. Letters in yellow indicate mismatches that are within the gRNA core (PAM-1 to PAM -14). Different colors in the code column indicate different pairs of homologous genes. C GmMLO gene structures and target locations, primers for genotyping are indicated by arrows. D T-DNA region for soybean transformation. bar, herbicide resistant gene as selection marker; pcoCas9, Cas9 codon-optimized gene, driven by 35SPPDK promoter (35Sp) and two sgRNAs driven by Arabidopsis U6 promoter (AtU6p); MASp, Manopine Synthase promoter; MASter, Manopine Synthase terminator; NOSter, Nopaline synthase terminator; LB/RB, Left and Right Border

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BMC Plant Biology

ISSN: 1471-2229

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