Fig. 3
From: Multiplex CRISPR-Cas9 knockout of EIL3, EIL4, and EIN2L advances soybean flowering time and pod set
Soybean triple mutation of EIL3, EIL4 and EIN2L generated by the CRISPR/Cas9 system in the T3 generation. A Sequences of WT and mutant plants at target sites EIL3-sg-T1, EIL4-sg-T2, and EIN2L-sg-T3. Dashes indicate deleted nucleotides. Proto-spacer adjacent motif (PAM) has been highlighted with red letters. B The sequence peaks of the wild-type (WT) and mutants at target sites EIL3-sg-T1, EIL4-sg-T2, and EIN2L-sg-T3. Red arrows indicate mutation locations. C Amino acid sequences of the triple mutation of EIL3, EIL4 and EIN2L and WT