Table 4 Effect of liquid colchicine medium on callus induced polyploidy
Colchicine Concentration (mg·L−1) | Duration (d) | Adventitious shoot induction rate (%) | Mixed ploidy induction rate (%) | Tetraploid induction rate (%) |
|---|---|---|---|---|
0 | 0 | 95.6 ± 2.2a | 0d | 0c |
150 | 1 | 91.1 ± 5.9ab | 0d | 0c |
150 | 2 | 88.9 ± 5.9abc | 4.7 ± 0.8bcd | 0c |
150 | 3 | 80.0 ± 7.7abcd | 7.0 ± 1.9bcd | 0c |
150 | 4 | 73.3 ± 3.8abcde | 8.3 ± 3.8bcd | 3.0 ± 1.5bc |
250 | 1 | 91.1 ± 4.4abc | 3.3 ± 0.7cd | 0c |
250 | 2 | 84.4 ± 9.7abcd | 5.7 ± 0.8bcd | 2.3 ± 1.2bc |
250 | 3 | 71.1 ± 13.5abcde | 9.0 ± 3.0bcd | 4.0 ± 2.0bc |
250 | 4 | 62.2 ± 8.9cdef | 11.7 ± 2.5bcd | 7.0 ± 4.1ab |
350 | 1 | 84.4 ± 12.4abcd | 7.3 ± 1.2bcd | 0c |
350 | 2 | 68.9 ± 8.9abcde | 15.3 ± 3.2bc | 10.0 ± 0.6a |
350 | 3 | 60.0 ± 10.2def | 31.7 ± 1.6a | 3.7 ± 3.7bc |
350 | 4 | 51.1 ± 9.7ef | 16.3 ± 8.5b | 0c |
450 | 1 | 64.4 ± 11.8bcdef | 12.3 ± 2.4bc | 5.0 ± 2.5abc |
450 | 2 | 57.8 ± 8.0def | 10.3 ± 5.4bcd | 0c |
450 | 3 | 48.9 ± 5.9ef | 6.7 ± 6.7bcd | 0c |
450 | 4 | 40.0 ± 6.7f | 0d | 0c |