Table 3 Effect of liquid colchicine medium on leaf induced polyploidy
Colchicine Concentration (mg·L−1) | Duration (d) | Adventitious shoot induction rate (%) | Mixed ploidy induction rate (%) | Tetraploid induction rate (%) |
|---|---|---|---|---|
0 | 0 | 95.6 ± 2.2a | 0b | 0b |
250 | 1 | 93.3 ± 3.8a | 0b | 0b |
250 | 2 | 86.7 ± 7.7ab | 1.3 ± 1.3b | 0b |
250 | 3 | 84.4 ± 9.7ab | 4.6 ± 2.4ab | 2.6 ± 2.6b |
250 | 4 | 77.8 ± 8.0ab | 6.1 ± 3.0ab | 3.3 ± 3.3b |
350 | 1 | 91.1 ± 5.9a | 3.7 ± 2.1b | 1.2 ± 1.2b |
350 | 2 | 84.4 ± 4.4ab | 4.9 ± 2.9ab | 3.2 ± 1.6b |
350 | 3 | 75.6 ± 5.9abc | 6.7 ± 3.4ab | 3.7 ± 3.7b |
350 | 4 | 68.9 ± 5.9bcd | 8.3 ± 8.3ab | 5.5 ± 5.6b |
450 | 1 | 84.4 ± 8.0ab | 8.0 ± 2.3ab | 4.3 ± 2.2b |
450 | 2 | 80.0 ± 3.8ab | 13.1 ± 2.6ab | 10.4 ± 6.5ab |
450 | 3 | 57.8 ± 2.2cd | 19.2 ± 4.8a | 18.7 ± 3.2a |
450 | 4 | 53.3 ± 3.8d | 11.1 ± 11.1ab | 8.3 ± 8.3ab |
550 | 1 | 82.2 ± 8.0ab | 7.0 ± 3.9ab | 4.4 ± 4.4b |
550 | 2 | 75.6 ± 4.4abc | 6.4 ± 3.2ab | 3.0 ± 3.0b |
550 | 3 | 55.6 ± 8.0d | 4.6 ± 4.8ab | 0b |
550 | 4 | 51.1 ± 5.9d | 0b | 0b |