Table 2 Effect of solid colchicine medium on callus induced polyploidy
Colchicine Concentration (mg·L−1) | Duration (w) | Adventitious shoot induction rate (%) | Mixed ploidy induction rate (%) | Tetraploid induction rate (%) |
|---|---|---|---|---|
0 | 0 | 95.6 ± 2.2a | 0b | 0 |
150 | 1 | 91.1 ± 5.9ab | 0b | 0 |
150 | 2 | 86.7 ± 3.8abc | 0b | 0 |
150 | 3 | 86.7 ± 3.8abc | 0b | 0 |
150 | 4 | 82.2 ± 4.4abcd | 0b | 0 |
250 | 1 | 88.9 ± 11.1ab | 0b | 0 |
250 | 2 | 86.7 ± 10.2abc | 0b | 0 |
250 | 3 | 80.0 ± 7.7abcd | 4.3 ± 2.1ab | 0 |
250 | 4 | 71.1 ± 8.0abcd | 10.8 ± 5.5a | 0 |
350 | 1 | 82.2 ± 9.7abcd | 0b | 0 |
350 | 2 | 75.6 ± 5.9abcd | 6.8 ± 0.9ab | 0 |
350 | 3 | 68.9 ± 2.2bcd | 9.26 ± 4.9ab | 0 |
350 | 4 | 60.0 ± 10.2d | 4.8 ± 4.8ab | 0 |
450 | 1 | 73.3 ± 6.7abcd | 4.6 ± 2.4ab | 0 |
450 | 2 | 68.9 ± 2.2bcd | 4.4 ± 4.4ab | 0 |
450 | 3 | 62.2 ± 14.6cd | 9.7 ± 5.8ab | 0 |
450 | 4 | 57.8 ± 8.9d | 0b | 0 |