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Table 1 Effect of solid colchicine medium on leaf induced polyploidy

From: Induction of tetraploids in Paper Mulberry (Broussonetia papyrifera (L.) L’Hér. ex Vent.) by colchicine

Colchicine Concentration (mg·L−1)

Duration (w)

Adventitious shoot induction rate (%)

Mixed ploidy induction rate (%)

Tetraploid induction rate (%)

0

0

95.6 ± 2.2a

0b

0b

250

1

93.3 ± 4.4ab

0b

0b

250

2

80.0 ± 3.8bcde

3.4 ± 1.7ab

0b

250

3

73.3 ± 10.2cdefg

4.2 ± 2.1ab

2.0 ± 2.0b

250

4

68.9 ± 2.2efgh

8.3 ± 8.3ab

6.7 ± 6.7ab

350

1

86.7 ± 6.7abc

3.1 ± 1.6ab

2.8 ± 1.4b

350

2

77.8 ± 2.2cdef

9.10 ± 2.2ab

6.9 ± 0.4ab

350

3

64.4 ± 4.4fgh

13.9 ± 3.2a

13.6 ± 2.4a

350

4

48.9 ± 2.2ij

11.1 ± 11.1ab

6.7 ± 6.7ab

450

1

84.4 ± 5.9abcd

7.7 ± 0.8ab

7.2 ± 3.7ab

450

2

75.6 ± 2.2cdefg

3.7 ± 3.7b

3.3 ± 3.3ab

450

3

62.2 ± 2.2ghi

0b

0b

450

4

44.4 ± 4.4jk

0b

0b

550

1

71.1 ± 2.2defg

5.6 ± 5.6ab

0b

550

2

55.6 ± 2.2hij

0b

0b

550

3

42.2 ± 4.4jk

0b

0b

550

4

31.1 ± 5.9k

0b

0b

  1. Each value represents the mean ± SE of three replicates, and means followed by the same letter in the same column are not significantly different from each other at P ≤ 0.05 level, according to Duncan’s multiple range test