Fig. 3

Phenotype analysis ofZm305

(A) Carrier structure of the Zm305 overexpression and CRISPR/Cas9 lines. (B) Expression pattern of Zm305. (C and D) Immature and mature kernel phenotypes of transgenic receptor, overexpression line, and two transgenic lines. (E and F) Comparison of the immature and mature kernel area of the transgenic receptor, overexpression line, and two transgenic lines. (G and H) Immature and mature kernel phenotypes of two reciprocal crosses that were used as a transgenic receptor hybrid with two transgenic lines. (I and J) Comparison of the immature and mature kernel area of two reciprocal crosses that were used as a transgenic receptor hybrid with two transgenic lines. The primers used in the mutant identification process are listed in Table S5. The error bars indicate ± SD. Significant differences were analyzed by two-tailed Student’s t tests (ns, not significant; *P < 0.05; **P < 0.01). The gRNA was short for guild RNA; DAP was short for day after pollination; FPKM was short for fragments per kilobase per million