Fig. 1

Identification and examples of ASIGs. (A) The pipeline to identify genes exhibiting allele-specific imprinting. A and B represent two inbred lines in maize. F1i (AB) represent that F1 hybrid from the inbred A (maternal line) crossing with inbred B (paternal line). F1r (BA) represent that F1 hybrid from the inbred B (maternal line) crossing with inbred A (paternal line). (B) The denoted abbreviation for ASIG showing maternal/paternal bias. A-MEG is maternally biased expression in A x B (AB), but is a no-biased expression in B x A (BA). B-MEG is a maternally biased expression in B x A (BA), but is no-biased expression in A x B (AB). A-PEG is paternally biased expression in B x A (BA), but is no-biased expression in A x B (AB). B-PEG is maternally biased expression in A x B (AB), but is no-biased expression in B x A (BA). (C) The number of ASIGs in the embryo and endosperm of three reciprocal hybrids (BC/CB, BM/MB and MC/CM). (D) The expression profile of four ASIGs identified in BC/CB endosperm. Gene expression levels are shown in green. The percentages of allelic reads for specific SNP sites are shown, with red lines for the paternal allele and blue lines for the maternal allele