Fig. 1

Barley SL insensitive mutant hvd14 is more sensitive to drought than the WT. A Overview of drought stress protocol used: plants were grown under optimal water conditions (15% of volumetric water content, vwc) for ten days after sowing (DAS). Next, plants were divided into two groups: control (C) plants that grew for another 15 days (25 DAS) under optimal water conditions and plants exposed to drought (D). For stressed plants, the watering was stopped for five days, which allowed to decrease VWC to 1.5–3%, and that value was maintained for another ten days. B Control and C. stressed plants at the end of the experiment (25 DAS). Effect of drought on D. shoot dry mass, E. shoot dry mass reduction, and F. relative water content (RWC) in leaves of both genotypes. Asterisks indicate statistically significant differences between samples in a paired Student's t-test (*, **, and *** correspond to p-values of 0.05 > p > 0.01, 0.01 > p > 0.001, and p < 0.001, respectively)