Fig. 4

Transcript expression of hypoxia-inducible genes in IR64-Sub1 seedlings after different treatments. Total RNA was isolated from shoots of 8-day old IR64-Sub1 seedlings after submergence (Sub), submergence with 300 mM mannitol (Sub + Man), submergence with 150 mM NaCl (Sub + NaCl) and control treatment (CK) for 8-day, respectively. The expression levels of (a) ADH1 and (b) LDH1 mRNA were determined by qRT-PCR. The data represented average values ± SD from four biologically independent experiments. Different letters indicate significant differences between treatments and cultivars (p < 0.05 according to an LSD using SAS)