Fig. 2

A-C: Interactive heatmap analysis of profiles and quantitative distribution of tentatively annotated metabolites in the control vs. Pst-infected Koonap cultivar. Figures A (flavonoids), B (amino acids, organic acids and indoles and derivatives) and C (lipids/fatty acids and benzenoids) show the relative quantification of annotated metabolites detected in Koonap samples over a four-week timepoint following infection. D-F: Interactive heatmap analysis of profiles and quantitative distribution of putatively annotated metabolites in the control vs. Pst-infected Gariep cultivar. Figures D (flavonoids), E (lipids/fatty acids and benzenoids) and F (amino acids, organic acids, indoles and derivatives) describe the relative quantification of annotated metabolites detected in Gariep samples over a four-week timepoint following infection. Up-regulated metabolites are shown in red whereas down-regulated metabolites are indicated by a green rating on the heatmap, while black shading shows no changes in the metabolite concentrations. The data projected above were median-normalised, log transformed and Pareto-scaled in MetaboAnalyst. The heatmap analysis thus showed the time-dependent changes in metabolic profiles of wheat plants due to pathogen infection compared to untreated plant samples