Fig. 3

Identification of the old12 mutation by whole-genome sequencing. A Genome-wide SNP frequency plot of the Arabidopsis genome using a bin size of 1 Mb for the old12 x Col-0 mapping population. Two candidate regions were identified for the old12 locus, one on chromosome I and one on chromosome III. B Genomic representation of the point mutation identified in P2K2 (AT3G45430). Light blue indicates exon sequence and gray corresponds to untranslated regions. C 32 DAS wild type (WT), old12 and two independent complementation (old12 gOLD12) lines grown under long-day photoperiod (16 h). Complementation of old12 with the genomic region containing AT3G45430 restores the wild-type phenotype. Scale bar = 1 cm. D Chlorophyll content (SPAD units) of the 3rd/4th leaf from WT, old12 and old12 gOLD12 #1. Each data point represents the mean ± SD from 10 leaves. E Ion leakage of the 3rd/4th leaf from WT, old12 and old12 gOLD12 #1. Each data point represents mean ± SD from 6 replicates of three leaves each. Asterisks indicate significant difference from WT (Student’s t-test; p ≤ 0.05)