Fig. 6From: Functional characterization of nine critical genes encoding rate-limiting enzymes in the flavonoid biosynthesis of the medicinal herb Grona styracifoliaExcavation and functional characterization on FNSII candidates from Grona styracifolia. (A) Phylogenetic tree of GsFNSII candidates. (B) Protein expression of GsFNSII during 48Â h of galactose induction. (C)In vivo enzymatic activity assay result of GsFNSII in Saccharomyces cerevisiae and mass spectrum of the enzymatic products apigenin, with yeast carrying the original vector served as the negative control. As in the mass spectrum, the number in red indicates the parental m/z and that in blue shows the major fragments. (D) Expression patterns of three GsFNSII genes in the roots, stems, and leaves of Grona styracifoliaBack to article page