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Fig. 5 | BMC Plant Biology

Fig. 5

From: Functional characterization of nine critical genes encoding rate-limiting enzymes in the flavonoid biosynthesis of the medicinal herb Grona styracifolia

Fig. 5

Excavation and functional characterization on CHI candidates from Grona styracifolia. (A) Phylogenetic tree of GsCHI candidates. (B) SDS-PAGE electrophoresis of recombinant GsCHI proteins, where lane 1 shows the total protein after induction, lane 2 shows the soluble protein, and lane 3 to 8 shows the purified recombinant proteins from the first to the sixth collected tube. (C) In vitro enzymatic activity assay result of GsCHI recombinant proteins and mass spectrum of the enzymatic products naringenin, with boiled protein and naringenin-chalcone served as the negative control. (D) In vitro enzymatic activity assay result of GsCHI recombinant proteins and mass spectrum of the enzymatic products liquiritigenin, with boiled protein and isoliquiritigenin served as the negative control. As in the mass spectrums, the number in red indicates the parental m/z and that in blue shows the major fragments. (E) Expression patterns of three GsCHI genes in the roots, stems, and leaves of Grona styracifolia

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BMC Plant Biology

ISSN: 1471-2229

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