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Fig. 4 | BMC Plant Biology

Fig. 4

From: Functional characterization of nine critical genes encoding rate-limiting enzymes in the flavonoid biosynthesis of the medicinal herb Grona styracifolia

Fig. 4

Excavation and functional characterization on CHS candidates from Grona styracifolia. (A) Phylogenetic tree of GsCHS candidates. (B) SDS-PAGE electrophoresis results for those GsCHS recombinant proteins, where lane 1 shows the total protein after induction, lane 2 shows the soluble protein, and lane 3 to 6 shows the purified recombinant proteins from the first to the fourth collected tube. (C) In vitro enzymatic activity assay result of GsCHS recombinant proteins and mass spectrum of the enzymatic products naringenin, with boiled protein and substrates served as the negative control. As in the mass spectrum, the number in red indicates the parental m/z and that in blue shows the major fragments. (D) Expression patterns of three GsCHS genes in the roots, stems, and leaves of Grona styracifolia

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BMC Plant Biology

ISSN: 1471-2229

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