Fig. 4

qRT-PCR analysis of seven cloned TPS genes in four hops cultivars during cone development. Cultivar abbreviations are given in Table 1. Gene expression data are mean ± SE, n = 3 and are given relative to reference gene DRH1 [37, 38]. Gene-specific primers are listed in Table S5. Samples at H0 were collected 10 days after flowering (DAF). H1-H6 were collected at around 10 d intervals from 20–70 DAF