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Fig. 4 | BMC Plant Biology

Fig. 4

From: Optimization of ATAC-seq in wheat seedling roots using INTACT-isolated nuclei

Fig. 4

Comparison of ATAC-seq data from wheat root tips and leaf protoplast. a Genomic distributions of enriched regions (peaks) identified in INTACT-ATAC-seq generated from fresh and frozen root tips compared with those from leaf protoplast (including only high confidence annotated genes in Chinese Spring Ref Seq v1.1 from A + B + unassigned chromosomes). b Venn diagram showing the overlap between INTACT-ATAC-seq peaks observed in fresh and frozen roots tissue, and in published leaf protoplast. The numbers of peaks detected with 5.6 M reads in both leaf and root (down-sampling Figure S2) is indicated in parenthesis. c Shot of ATAC-seq data for a 30-kb region of the Chinese Spring wheat genome from INTACT-purified nuclei from frozen and fresh root tissue and reanalyzed published leaf protoplast data

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