Fig. 3

TAD dynamics during cold stress in Bd21. A Overlap of TAD boundaries in normal and cold-treated Bd21. Consensus borders were defined by allowing a shift of two bins (10 kb) in each direction between the two conditions. B Comparison of insulation scores between the conserved and specific boundaries in each sample. C Gene Ontology (GO) biological process analysis of genes in specific TAD boundaries. The top ten enriched GO biological processes are indicated. D The proportion of differentially expressed genes in specific TAD boundaries. The overall prevalence of DEGs is the control group (background). GO enrichment analysis of differentially expressed genes in specific TAD boundaries was performed. The top ten enriched GO biological processes are indicated. E Relative proportion of upregulated and downregulated DEGs in normal- and cold-specific TAD boundaries, respectively. The overall DEGs are the control group (background). F An example of a TAD boundary, which is gained in cold stress, can be observed in a region on chromosome 2 (grey region). TADs identified as regions of high chromatin interactions are outlined by black triangles