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Fig. 5 | BMC Plant Biology

Fig. 5

From: Determination of superior Pistacia chinensis accession with high-quality seed oil and biodiesel production and revelation of LEC1/WRI1-mediated high oil accumulative mechanism for better developing woody biodiesel

Fig. 5

Isolation of PcWRI1 and PcLEC1 genes and genetic transformation of Arabidopsis. a Cloning of PcWRI1 gene from P. chinensis seeds by PCR (M: DNA marker; 1: PcWRI1 gene fragment). b Cloning of PcLEC1 gene from P. chinensis seeds by PCR (M: DNA marker; 1: PcLEC1 gene). c Illustration of binary vector of pCAMBIA/35S::PcWRI1 or pCAMBIA/35S::PcLEC1. d Verification of pCAMBIA/35S::PcWRI1 construction by restriction enzyme-digested assay (M1: DNA marker; 1: single enzyme-digested product by EcoR I; 2: double enzyme-digested product by EcoR I and BamH I; M2: DNA marker). e Verification of pCAMBIA/35S::PcLEC1 construction by restriction enzyme-digested assay (M1: DNA marker; 1: single enzyme-digested product by Xba I; 2: double enzyme-digested product by Xba I and Kpn I; M2: DNA marker). f Resistance screening of PcWRI1 transgenic Arabidopsis. g Resistance screening of PcLEC1 transgenic Arabidopsis. h Verification of integration of PcWRI1 within genome of Arabidopsis lines by PCR detection (M: DNA marker; CK-: WT negative control; 1–5: PcWRI1 transgenic lines; CK + : vector positive control). i Verification of integration of PcLEC1 within genome of Arabidopsis by PCR assay (M: DNA marker; CK-: WT negative control; 1–5: PcLEC1 transgenic lines; CK + : vector positive control). The full-length gels are presented in Additional file 4: Fig. S1

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BMC Plant Biology

ISSN: 1471-2229

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