Fig. 4

Seven melon-associated metabolites in aphids during fasting. C6 adult aphids were reared on Védrantais melon plants, then fasted by batches of 20 to 50 individuals for 3 h, 6 h and 23 h. t0 corresponds to the aphids sampled at rearing (before fasting). For each time point, four aphid batches were sampled, except for the “3 h” point where three aphid batches were sampled. Stachyose, sucrose and galactinol were analysed by GC–MS in aphid batches and expressed as peak area (no units) corrected by the aphid batch weight (mg). Cucurbitacin B and Glycosylated Cucurbitacin B were not detected by LC–MS. The metabolite contents in aphid batches at t = 3 h, 6 h and 23 h were compared to t0 using a unilateral non-parametric Kruskal–Wallis test corresponding to alpha = 0.05 (i.e. alpha = 0.025). Only significant differences are marked in the figure