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Fig. 3 | BMC Plant Biology

Fig. 3

From: Aphid adaptation to cucurbits: sugars, cucurbitacin and phloem structure in resistant and susceptible melons

Fig. 3

Quantification of five phloem-associated metabolites in healthy versus aphid-infested melon plants. VĆ©drantais and its isogenic [VĆ©drantais]R_PI 161375 plants were infested for 17Ā h by NM1 aphid clone -triggering the Vat-mediated resistance- in both experiments, and by either GWD2 aphids -triggering too the Vat-mediated resistance- in experiment (1) or C6 aphids -non-triggering the Vat-mediated resistance in experiment (2). Stachyose, sucrose and galactinol were analysed by GCā€“MS in melon leaves and expressed as peak area (no units) corrected by leaf sample weight (g). Cucurbitacin B and Glycosylated Cucurbitacin B were analysed by LCā€“MS and expressed as nMole.mgāˆ’1. The number of observations per melon genotype was between 6 and 9. Statistical comparisons were made between infestation conditions for a melon genotype (letter codes indicate significant differences) using a non- parametric bilateral Kruskalā€“Wallis test at alphaā€‰=ā€‰0.05

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