Fig. 4From: Building an improved transcription factor-centered yeast one hybrid system to identify DNA motifs bound by protein comprehensivelyEMSA verification of the bindings of the selected motifs with BpERF2. The eight selected study motifs were used for EMSA analysis. The competitor for the labeled probe was tested by adding 20, 50, and 100-fold excess of the same unlabeled probe. The biotin-labeled, mutated probes were used as the negative control. Arrows indicate the free probes and the DNA-protein complexesBack to article page