Fig. 7

Exogenous melatonin treatment decreases CGMMV accumulation in cucumber and has preventive effect on other Tobamovirus. A Symptoms of early stage CGMMV infection at 17 dpi on cucumber leaves. CGMMV CP accumulation in systemically infected cucumber leaves at 17 dpi was examined using qRT-PCR (B) and Western blotting (C) analysis with CGMMV CP antibody. Full-length blots/gels are presented in Supplementary Fig. 7. D PMMoV-GFP infection at 7 dpi in tobacco leaves treated by melatonin. PMMoV accumulation in systemically infected leaves at 7 dpi was examined using qRT-PCR (E) and western blotting (F) analysis with GFP antibody. Full-length blots/gels are presented in Supplementary Fig. 8. Bars are SEM from three biological repeats. Significant differences were evaluated using a two-sample unequal variance t-test (*P < 0.05; **P < 0.01)