Fig. 5
CRISP1 silencing enhanced melatonin-induced CGMMV prevention responses. A Systemic infection by CGMMV at 8 dpi in plants pre-treated with TRV:00 or TRV:CRISP1. TRV:00/MEL and TRV:CRISP1/MEL plants were irrigated continuously with melatonin for 3 days prior to CGMMV infection; ddH2O was used as a control. CGMMV CP accumulation in systemically infected leaves at 9 dpi was examined using (B) qRT-PCR and (C) western blotting with CGMMV CP antibody. Bars are SEM from three biological repeats. Significant differences were evaluated using a two-sample unequal variance t-test (*P < 0.05; **P < 0.01). Full-length blots/gels are presented in Supplementary Fig. 6