Fig. 4

Expression Analysis of the McWRKY57-like gene under different treatments. A and B Time-course expression levels of McWRKY57-like in leaves and adventitious roots under treatment with 300 mM mannitol. C and D Time-course expression levels of McWRKY57-like in leaves and adventitious roots under treatment with 150 mM NaCl. E and F Time-course expression levels of McWRKY57-like in leaves and adventitious roots under treatment with 100 µM ABA. G and H Time-course expression levels of McWRKY57-like in leaves and adventitious roots under treatment with 200 µM MeJA. For each treatment, McACT was used as the reference gene, and expression values are relative to that of 0 h, and data represented means ± SE of three replicates. Different letters indicate significant differences at P < 0.05, as determined by one-way analysis of variance (ANOVA) with Tukey’s post-test. I Expression profiles of the McWRKY57-like gene in leaves of M. canadensis under natural drought stress. McACT was used as the reference gene, and the expression level was normalized to the untreated control. The asterisk (*) indicated significant differences at P < 0.05, as determined by one-way analysis of variance (ANOVA) with Tukey’s post-test